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Total Internal Reflection Microscopy

См. также в других словарях:

  • Total internal reflection fluorescence microscope — (TIRFM) diagram 1. Specimen 2. Evanescent wave range 3. Cover slip 4. Immersion oil 5. Objective 6. Emission beam (signal) 7. Excitation beam ] 1. Objective 2. Emission beam (signal) 3. Immersion oil 4. Cover slip 5. Specimen 6. Evanescent wave… …   Wikipedia

  • Microscopy — is the technical field of using microscopes to view samples and objects that cannot be seen with the unaided eye (objects that are not within the resolution range of the normal eye). There are three well known branches of microscopy, optical,… …   Wikipedia

  • Supercritical angle fluorescence microscopy — (SAF) is a technique to detect and characterize fluorescent species (proteins, biomolecules, pharmaceuticals, etc.) and their behaviour very close or even adsorbed or linked at surfaces. The method is able to observe molecules in a distance of… …   Wikipedia

  • Confocal microscopy — Diagnostics MeSH D018613 OPS 301 code 3 301 …   Wikipedia

  • Dark field microscopy — (dark ground microscopy) describes microscopy methods, in both light and electron microscopy, which exclude the unscattered beam from the image. As a result, the field around the specimen (i.e. where there is no specimen to scatter the beam) is… …   Wikipedia

  • Confocal laser scanning microscopy — (CLSM or LSCM) is a technique for obtaining high resolution optical images with depth selectivity.[1] The key feature of confocal microscopy is its ability to acquire in focus images from selected depths, a process known as optical sectioning.… …   Wikipedia

  • Two-photon excitation microscopy — is a fluorescence imaging technique that allows imaging of living tissue up to a very high depth, that is up to about one millimeter. Being a special variant of the multiphoton fluorescence microscope, it uses red shifted excitation light which… …   Wikipedia

  • Differential interference contrast microscopy — Micrasterias furcata imaged in transmitted DIC microscopy. Differential interference contrast microscopy (DIC), also known as Nomarski Interference Contrast (NIC) or Nomarski microscopy, is an optical microscopy illumination technique used to… …   Wikipedia

  • Phase contrast microscopy — Phase contrast image of a cheek epithelial cell Phase contrast microscopy is an optical microscopy illumination technique of great importance to biologists in which small (invisible to the human eye) phase shifts in the light passing through a… …   Wikipedia

  • Multifocal multiphoton microscopy — is a microscopy technique for generating 3D images. By exploiting specific properties of pulsed mode multiphoton excitation the conflict between the density of the foci, i.e. the degree of parallelization, and the axial sectioning has been… …   Wikipedia

  • Optical sectioning — is the process by which a suitably designed microscope can produce clear images of a focal planes deep within a thick sample. This is used to reduce the need for thin sectioning using instruments such as the microtome. Many different techniques… …   Wikipedia

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